Review



rb mab anti ha  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc rb mab anti ha
    Rb Mab Anti Ha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/pmc12844901-381-5-8
    Average 86 stars, based on 1 article reviews
    rb mab anti ha - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
    Article Snippet: Weekly subculture was performed by removing growth media, adding cold Gentle Dissociation Reagent (StemCell Technologies 07174), and pipetting until matrigel was dissociated from the plastic and then pipetted again until organoids were broken open to release dead cells from the organoid lumen. .. Rb-anti-HA (Cell Signaling, Clone C29F4, Cat3724) or Rb-anti-V5 (Cell Signaling, Clone D3H8Q, Cat#13202 s) were diluted 1:500 in FISHX and incubated overnight at 4 °C overnight, washed, and then blocked in FISHX with 5% Normal Goat Serum (NGS, Thermofisher 1610064) for 30 min. Tissues were then stained for 1.5 h with Alexa FluorTM-633 goat anti-rabbit (Thermofisher A21070) diluted 1:500 in FISHX/5%NGS. .. For FLAG immunostaining, Ms-IgG1-anti-FLAG (Sigma, F1804) was used at 1:250 and the protocol was similar to HA and V5 immunostaining but with the inclusion of a mouse-on-mouse blocking reagent (Jackson Immuno Research Laboratories, AffiniPure Fab Fragment Donkey Anti-Mouse IgG, 715-007-003) during tissue permeabilization and appropriate secondary antibody (Alexa FluorTM-633 goat anti-mouse IgG(g1), ThermoFisher A21126).

    Next-Generation Sequencing:

    Article Title: A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
    Article Snippet: Weekly subculture was performed by removing growth media, adding cold Gentle Dissociation Reagent (StemCell Technologies 07174), and pipetting until matrigel was dissociated from the plastic and then pipetted again until organoids were broken open to release dead cells from the organoid lumen. .. Rb-anti-HA (Cell Signaling, Clone C29F4, Cat3724) or Rb-anti-V5 (Cell Signaling, Clone D3H8Q, Cat#13202 s) were diluted 1:500 in FISHX and incubated overnight at 4 °C overnight, washed, and then blocked in FISHX with 5% Normal Goat Serum (NGS, Thermofisher 1610064) for 30 min. Tissues were then stained for 1.5 h with Alexa FluorTM-633 goat anti-rabbit (Thermofisher A21070) diluted 1:500 in FISHX/5%NGS. .. For FLAG immunostaining, Ms-IgG1-anti-FLAG (Sigma, F1804) was used at 1:250 and the protocol was similar to HA and V5 immunostaining but with the inclusion of a mouse-on-mouse blocking reagent (Jackson Immuno Research Laboratories, AffiniPure Fab Fragment Donkey Anti-Mouse IgG, 715-007-003) during tissue permeabilization and appropriate secondary antibody (Alexa FluorTM-633 goat anti-mouse IgG(g1), ThermoFisher A21126).

    Staining:

    Article Title: A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion
    Article Snippet: Weekly subculture was performed by removing growth media, adding cold Gentle Dissociation Reagent (StemCell Technologies 07174), and pipetting until matrigel was dissociated from the plastic and then pipetted again until organoids were broken open to release dead cells from the organoid lumen. .. Rb-anti-HA (Cell Signaling, Clone C29F4, Cat3724) or Rb-anti-V5 (Cell Signaling, Clone D3H8Q, Cat#13202 s) were diluted 1:500 in FISHX and incubated overnight at 4 °C overnight, washed, and then blocked in FISHX with 5% Normal Goat Serum (NGS, Thermofisher 1610064) for 30 min. Tissues were then stained for 1.5 h with Alexa FluorTM-633 goat anti-rabbit (Thermofisher A21070) diluted 1:500 in FISHX/5%NGS. .. For FLAG immunostaining, Ms-IgG1-anti-FLAG (Sigma, F1804) was used at 1:250 and the protocol was similar to HA and V5 immunostaining but with the inclusion of a mouse-on-mouse blocking reagent (Jackson Immuno Research Laboratories, AffiniPure Fab Fragment Donkey Anti-Mouse IgG, 715-007-003) during tissue permeabilization and appropriate secondary antibody (Alexa FluorTM-633 goat anti-mouse IgG(g1), ThermoFisher A21126).



    Similar Products

    86
    Cell Signaling Technology Inc rb mab anti ha
    Rb Mab Anti Ha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/pmc12844901-381-5-8
    Average 86 stars, based on 1 article reviews
    rb mab anti ha - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc rb anti ha
    Rb Anti Ha, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/HA-Tag+Rabbit+mAb/pm40120584-310-7-8
    Average 99 stars, based on 1 article reviews
    rb anti ha - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    90
    Millipore rabbit (rb) anti-ha antibody
    Rabbit (Rb) Anti Ha Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/rabbit+anti+ha/pmc11087495-369-0-4
    Average 90 stars, based on 1 article reviews
    rabbit (rb) anti-ha antibody - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    86
    Danaher Inc rb anti ha antibody
    Rb Anti Ha Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/us11958884-211-4-7
    Average 86 stars, based on 1 article reviews
    rb anti ha antibody - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc rb anti ha
    Rb Anti Ha, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rb+anti+ha/pmc10753533-71-10-11
    Average 86 stars, based on 1 article reviews
    rb anti ha - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    Image Search Results